My project focuses on understanding how the parasite Toxoplasma gondii grows and divides at the cellular level. Toxoplasma infects nearly a third of the global population and can cause serious disease in pregnant individuals and people with weakened immune systems. Instead of studying the behavioral effects the parasite is famous for, my work looks at how it physically builds new daughter cells inside the mother cell; a unique form of replication that depends on structural genes like SFA2 and SFA3. These genes act as internal scaffolding that shape the parasite and organize its cell division. In the lab, I use molecular cloning techniques: PCR, Gibson assembly, and plasmid construction to isolate, copy, and modify these gene sequences. The goal of the project is to map how these structural components contribute to parasite division, which helps identify vulnerable steps in its life cycle that future treatments could target.